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Effect of DSS serum on apoptosis of HT-22. Cells were divided into five groups: HT-22 (H), HT-22+STZ (S), HT-22+STZ+DSS (D),HT-22+STZ <t>+MCUi4(M),</t> HT-22+STZ+DSS+MCUi4 (D+M). ( A ) Results of Western blot. ( B ) Protein expression results of Bax/Bcl-2(n = 3). ( C ) Results of Western blot. ( D ) Protein expression results of p-CaMKII(n = 3). ( E ) Protein expression results of cyt-c(n = 3). ( F ) Protein expression results of cl-Caspase-3 (n = 3). ( G ) Results of Western blot. ( H )Protein expression results of MCU (n = 3). ( I )Apoptosis by flow cytometry. ( J )Apoptosis rate(n = 3). *indicates P < 0.05; **indicates P < 0.01; *** indicates P < 0.001; **** indicates P < 0.0001; ns indicates no statistical difference.
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Effect of DSS serum on apoptosis of HT-22. Cells were divided into five groups: HT-22 (H), HT-22+STZ (S), HT-22+STZ+DSS (D),HT-22+STZ +MCUi4(M), HT-22+STZ+DSS+MCUi4 (D+M). ( A ) Results of Western blot. ( B ) Protein expression results of Bax/Bcl-2(n = 3). ( C ) Results of Western blot. ( D ) Protein expression results of p-CaMKII(n = 3). ( E ) Protein expression results of cyt-c(n = 3). ( F ) Protein expression results of cl-Caspase-3 (n = 3). ( G ) Results of Western blot. ( H )Protein expression results of MCU (n = 3). ( I )Apoptosis by flow cytometry. ( J )Apoptosis rate(n = 3). *indicates P < 0.05; **indicates P < 0.01; *** indicates P < 0.001; **** indicates P < 0.0001; ns indicates no statistical difference.

Journal: Drug Design, Development and Therapy

Article Title: Danggui-Shaoyao-San Can Ameliorate Alzheimer’s Disease by Inhibiting Hippocampal Neuron Apoptosis: Findings from Serum Pharmacology

doi: 10.2147/DDDT.S490900

Figure Lengend Snippet: Effect of DSS serum on apoptosis of HT-22. Cells were divided into five groups: HT-22 (H), HT-22+STZ (S), HT-22+STZ+DSS (D),HT-22+STZ +MCUi4(M), HT-22+STZ+DSS+MCUi4 (D+M). ( A ) Results of Western blot. ( B ) Protein expression results of Bax/Bcl-2(n = 3). ( C ) Results of Western blot. ( D ) Protein expression results of p-CaMKII(n = 3). ( E ) Protein expression results of cyt-c(n = 3). ( F ) Protein expression results of cl-Caspase-3 (n = 3). ( G ) Results of Western blot. ( H )Protein expression results of MCU (n = 3). ( I )Apoptosis by flow cytometry. ( J )Apoptosis rate(n = 3). *indicates P < 0.05; **indicates P < 0.01; *** indicates P < 0.001; **** indicates P < 0.0001; ns indicates no statistical difference.

Article Snippet: The MCU is blocked by treating HT-22 cells with 10μM MCUi4(HY-138620, MCE).

Techniques: Western Blot, Expressing, Flow Cytometry